Hauj lwm lawm

Kev tsis

Taw qhia

IGF-DES2

musclepic 3

Khoom Description

Ntu 1: Cov yam ntxwv ntawm tus yam ntxwv thiab Molecular Basis

1. Amino Acid Sequence thiab Molecular Conformation

IGF-DES yog tsim los ntawm kev tshem tawm thawj peb cov amino acids los ntawm N-terminus ntawm IGF-1 molecule, uas ua rau tag nrho ntev ntawm 67 amino acids.

Qhov kev hloov pauv amino acid no ua rau muaj cov yam ntxwv hauv qab no:

Qhov luv N-terminus ua rau nws yooj yim dua los khi rau IGF-1 receptor (IGF-1R).

Nws ua rau tsis muaj zog ntawm kev sib txuas rau IGF-binding proteins (IGFBPs).

Lub molecule nthuav tawm ntau dua kev ua haujlwm lom hauv zos.

Nws yooj yim exerts nws cov teebmeem nyob ib ncig ntawm lub hom phiaj cov ntaub so ntswg.

Tus qauv ntawm IGF-DES khaws cov -daim ntawv thiab peb- lub nplhaib sib txuas ntawm IGF-1 niam txiv molecule, tab sis nws lub davhlau ya nyob twg hloov pauv tso cai rau kev hloov pauv yooj yim dua.

2. Molecular Luj thiab tshuaj muaj zog

Qhov hnyav molecular yog kwv yees li 7.5 kDa.

Nws yog hydrophilic protein peptide, yooj yim soluble nyob rau hauv ib co weakly acidic tsis daws teeb meem.

Nws muaj qhov qis isoelectric point, ua rau nws ruaj khov nyob rau hauv acidic ib puag ncig.

Qhov me me molecular hnyav piv rau qhov tsis zoo IGF-1 ntxiv txhim kho nws cov ntaub so ntswg nkag thiab ua haujlwm hauv zos.

Tshooj 2 Physicochemical Performance Analysis

1. Cov tsos thiab xim

High-kev tshawb fawb zoo-qib IGF-DES raw khoom yog feem ntau:

Dawb kom tawm -dawb lyophilized hmoov

Fine thiab uniform hmoov

Yuav tsum tsis txhob yellowing, browning, los yog pom tseeb clumping.

Lyophilization tshuab ua kom nws cov qauv ruaj khov thiab tiv thaiv peptide saw degradation.

Kev khaws cia tsis raug ua rau oxidation lossis noo noo nqus tuaj yeem ua rau xim tsaus me ntsis.

2. Solubility Properties

Raws li hydrophilic peptide siab heev, IGF-DES:

Soluble nyob rau hauv sterile dej thiab qaug zog acid buffer daws

Stability txo qis hauv nruab nrab lossis alkaline ib puag ncig

Cov khoom siv raw hmoov yuav tsum tau tiv thaiv los ntawm kev raug ncaj qha rau cov dej noo.

Nws lub xeev daws tau yooj yim cuam tshuam los ntawm qhov kub thiab txias, pH, thiab enzymatic hydrolysis; yog li ntawd, nws yog feem ntau npaj thiab siv tam sim ntawd hauv kev tshawb fawb thwmsim.

3. Cov yam ntxwv ruaj khov thiab khaws cia

IGF-DES nthuav tawm kev ruaj ntseg zoo hauv nws lub xeev lyophilized:

Khaws rau hauv qhov chaw qhuav, lub teeb - tiv thaiv ib puag ncig ntawm -20℃thiab -80℃.

Tsis txhob rov khov dua -thaw cycles.

Relatively ruaj khov nyob rau hauv acidic tsis muaj zog buffer daws.

Photosensitive; yuav tsum khaws cia deb ntawm lub teeb.

Kev kub siab tuaj yeem ua rau peptide saw degradation, thaum nce av noo tuaj yeem ua rau muaj kev sib sau los yog kev hloov pauv.

Tshooj 3 Kev Tswj Xyuas Zoo thiab Kev Ntsuas Cov Qauv

Txhawm rau kom ntseeg tau qhov zoo ntawm kev tshawb fawb-qib IGF-DES, yuav tsum tau kuaj xyuas kom zoo, suav nrog:

1. Kev tsom xam Purity

Mainstream analytical txoj kev:

HPLC (High Performance Liquid Chromatography)

LC-MS (Liquid Chromatography-Mass Spectrometry)

MALDI-TOF Mass Spectrometry

High - cov khoom zoo feem ntau xav tau tus qauv purity ntawm Ntau dua lossis sib npaug li 98%.

2. Molecular Nyhav Pom Zoo

Mass spectrometry yog siv los xyuas kom meej tias qhov hnyav molecular phim tus nqi theoretical, zam impurities xws li truncated peptides thiab oxidized peptides.

3. Bioactivity-Txoj Kev Ntsuam Xyuas (Kev Tshawb Fawb Kev Tshawb Fawb)

Xws li:

IGF-1R Binding Capacity Assessment

Kev ntsuam xyuas lub zog ntawm PI3K/Akt Pathway Regulation

Cell Survival thiab Proliferation Assay

Cov kev ntsuam xyuas no yog rau kev tshawb fawb nkaus xwb thiab tsis yog rau txhua daim ntawv thov kho mob.

4. Kev kuaj xyuas Endotoxin

High-zoo IGF-DES yuav tsum dhau:

LAL (Limulus Amebocyte Lysate) kev ntsuam xyuas rau qib endotoxin

Xyuas kom ua raws li kev tshawb fawb -qib lossis cov qauv tsis muaj menyuam

Ntu 4: Cov txiaj ntsig tseem ceeb ntawm IGF-DES (Kev Tshawb Fawb Txog Kev Tshawb Fawb)

Los ntawm kev tshawb fawb pom, IGF-DES muaj cov yam ntxwv zoo hauv qab no:

1. Kev ua haujlwm hauv zos siab dua

Vim qhov txo qis ntawm IGFBP kev sib khi muaj peev xwm, IGF-DES muaj qhov siab dua dawb nyob hauv cov ntaub so ntswg hauv zos, ua kom yooj yim rau khi rau IGF-1R thiab ua rau muaj teeb meem.

2. Kev ua kom muaj teeb meem ceev dua

IGF-DES, thaum khi rau nws cov receptor, tuaj yeem qhib:

PI3K/Akt Pathway

MAPK/ERK Txoj Kev

Cov no yog txoj hauv kev rau kev kawm txog kev loj hlob ntawm tes, sib txawv, kho, thiab metabolism.

3. Cov ntaub so ntswg muaj zog

Luv luv molecules thiab curvature siab dua muab nws superior nkag mus rau IGF-1 tsis zoo.

4. Haum rau ntau yam kev tshawb fawb qauv

Xws li:

Kev sim ntawm Cell Proliferation

Cov ntaub so ntswg Regeneration Models

Metabolic Pathway Studies

Growth Factor Kinetic Analysis

Local Stimulus-Cov qauv muaj feem xyuam

5. Yooj Yim Khov -Kev ziab thiab khaws cov khoom siv raw

Cov qauv ruaj khov, ua kom yooj yim rau lub sijhawm ntev - khaws cia tom qab khov - ziab.

Ntu 5 Cov txheej txheem tsim khoom thiab cov yam ntxwv ntawm cov khoom siv raw

Siab -zoo peptide IGF-DES feem ntau yog tsim los siv Khoom-Phase Peptide Synthesis (SPPS):

1. Theem Synthesis

Siv cov khoom siv resin

Txuas cov peptide saw amino acid los ntawm amino acid

Txo qhov tsis sib haum xeeb thiab truncation

2. Cleavage thiab Purification theem

TFA cleavage

Ntau qhov kev lim dej

HPLC purification

Sau lub ntsiab peptide ncov

3. Qib Lyophilization

Cov tshuaj purified peptide tau dhau los:

Tsawg-kub khov

Nqus sublimation

Tau txais cov hmoov dawb lyophilized

4. Kev kuaj zaum kawg

Nrog rau kev pom zoo ntawm cov qauv, kev kuaj purity, kev tshawb nrhiav cov kuab tshuaj residual, thiab kev tshawb nrhiav colony.

Ntu 6 Kev Nyab Xeeb Kev Nyab Xeeb (Kev Tshawb Fawb Kev Pom Zoo)

Raws li kev tshawb fawb -qib raw khoom peptide, kev nyab xeeb ntawm IGF-DES suav nrog:

rhiab heev rau qhov kub thiab txias

Heev yooj yim decomposed los ntawm proteases

Tsawg stability nyob rau hauv kev daws

Tsis yog siv los ua tshuaj lossis tshuaj kho mob

Nws daim ntawv thov yuav tsum txwv rau qhov chaw kuaj thiab ua haujlwm los ntawm cov kws tshawb fawb.

Ntu 7 Summary

Raws li kev txiav tawm derivative peptide ntawm IGF-1, IGF-DES muaj qhov tshwj xeeb zoo hauv cov qauv, molecular zog, receptor affinity, thiab kev ua haujlwm hauv zos. Raws li kev tshawb fawb qib siab peptide, nws tau siv dav hauv ntau yam kev tshawb fawb xws li cell biology, teeb liab hloov, thiab kho cov ntaub so ntswg.

Nws muaj cov yam ntxwv hauv qab no:

Cov qauv luv tab sis nquag nquag

Dawb lyophilized hmoov, yooj yim khaws cia

Highly hydrophilic, soluble nyob rau hauv weakly acidic aqueous daws

Kev ruaj ntseg nruj thiab kev cia khoom