Lub hauv paus rau phau ntawv

HGH 2

Factory 2

musclepic 1

Cov khoom lag luam

#### ** 1. Cov Ntsiab Lus Txhais thiab Qhia Txog: Dab tsi yog somatropin (amino acid 191)? **

Kom nkag siab Hghropin, peb yuav tsum xub hais meej lub tswvyim tseem ceeb: nws yog qhov sib txawv ntawm lwm cov khoom keeb kwm, "Somatropin." Somatropin yog kev loj hlob muaj kev sib raug zoo ntawm 192 amino acids. Qhov no cov amino acid ua nws cov qauv molecular me ntsis sib txawv los ntawm endogenous tib neeg kev loj hlob lawm. Qhov no tau pom hauv kev siv tshuab thev naus laus zis DNA, tab sis vim nws cov kev txhaj tshuaj tiv thaiv siab (muaj peev xwm ua rau tshuaj tiv thaiv hauv lub cev), nws tau raug tshem tawm ntau.

Cov ntsiab lus meej ntawm Somatropin, uas peb yuav ua kom pom tseeb rau hauv cov kab kev sib tw ua ke xws li Eschrehicide ** Tus lej "191" yog qhov tseem ceeb, ntaus, hom, thiab xaj cov amino acids muaj txiaj ntsig zoo rau tib neeg cov qog pituitary. Cov qauv txheej txheem no yog cov khoom siv rau nws lub peev xwm los ua kom raug rau txhua yam kev ua si ntawm HGH thiab yog qhov lav tau ntawm nws kev nyab xeeb thiab kev ua tau zoo. Yog li ntawd, SomaRopin (amino acid 191) yog qhov qauv, tag nrho biologically nquag recombable tib neeg txoj kev loj hlob hormone.

#### ** 2. Cov ncauj lus kom ntxaws txog lub cev: molecular - tus yam ntxwv **

Raws li cov protein loj, Somatropin cov khoom siv dag ntxias txiav txim siab txog nws qhov ruaj khov, kev cia khoom, thiab kev tswj hwm.

** 1. Molecular qauv thiab ruaj khov: **

Somatropin yog polypeptide saw muaj ntawm 191 amino acids txuas los ntawm peptide bonds. Cov qauv molecule ua cov qauv theem nrab thiab qib siab rau qib, suav nrog ntau- helsia. Tus qauv ntse no yog qhov tseem ceeb rau nws cov haujlwm, tab sis nws kuj ua rau nws rhiab rau cov chaw sab nraud. Protein ruaj khov yog cov tub ntxhais tus tseem ceeb ntawm lub cuab yeej, encompassing nws muaj peev xwm los tswj nws cov kev sib txuas lus thiab roj ntsha.

** 2. Kub rhiab: **

Txom nyem thermal stability yog ib tug cwj pwm nquag yog cov protein ntau tshaj, thiab Somaropin tsis muaj kev zam. Kev kub siab yog nws qhov kev hem thawj thawj zaug. Feem ntau, unpenenen Somatropin Lyophilized hmoov yuav tsum muab khaws cia rau ntawm 2℃thiab 8℃los tswj kom ntev {4 4}}} lub sij hawm ruaj khov. Thaum raug rau chav sov, lossis tshwj xeeb tshaj yog rau kev nce qib kub (piv txwv li 25 degree), xws li hydrogen bonds thiab hydrophobic sib cuam tshuam) cuam tshuam, ua rau protein denaturation. Denatured Somatropin hloov pauv los ntawm nws cov neeg cog lus sib xyaw mus rau txoj kev xoob xoob, random coil qauv, mus tas li poob nws cov kev ua si. Rov kho dua (yaj) cov kev daws teeb meem yog qhov ntsuas kub ntau dua {}}} rhiab nyob rau hauv tub yees (piv txwv li, tsis pub dhau 24 teev) los tiv thaiv kev ua haujlwm poob thiab microbial kev loj hlob.

3. PHEEJ YEEM:

Somatropin's Solubility thiab Ruaj Ntseg yog heev nyob ntawm PH ntawm cov kev daws teeb meem uas nws khaws cia. Nws cov solubility yog qhov qis tshaj ze nws lub ISOELECTRIC PARCH (PI), ib txwm nyob ib ncig ntawm PH.0. Nws solubility nce nyob rau hauv me ntsis acidic lossis alkaline tej yam kev mob. Txawm li cas los xij, theem qib siab (overly acidic lossis overly alkaline) kuj tseem tuaj yeem ua rau cov qauv ua kom puas thiab tsis ua haujlwm. Yog li no, cov lag luam somatropin cov khoom lag luam feem ntau yuav rov ua kom haum rau cov kuab tsw qab (feem ntau yog cov kua qaub) los muab cov dej ua kua zoo rau nws lub zog.

** 4. Solubility thiab daim ntawv: **

Hauv kev ua lag luam ua tiav, kom ua tiav lub neej ua kom tiav, tshem tawm cov protein ntau, ua tiav kom qhuav thiab tshem tawm cov khoom ruaj khov thiab ua kom ruaj khov rau ob peb xyoo hauv qab. Thaum rov nrog cov dej nrog cov dej tsis huv rau kev txhaj tshuaj lossis cov hmoov tsis zoo, cov ntawv sau ua tsis muaj kob kom me ntsis rau cov xim ntshiab daj.

#### ** 3. Kev thov koj zoo dua thiab cov nqi kho mob **

Qhov tseem ceeb tshaj plaws ntawm Somatropin (amino acid 191) nyob hauv nws cov homology ua tiav kom tiav rau cov neeg muaj cov tshuaj hormones uas yog qhov kev thov tsis tau zoo.

** 1. Kev nyab xeeb siab thiab tsis muaj kev tiv thaiv kab mob siab: **

Raws li tau hais los saum toj no, vim hais tias nws cov qauv yog ib qho zoo ib yam rau kev siv HGH, cov tib neeg lub cev tsis muaj zog lees paub nws li "tus kheej" es tsis yog "txawv teb chaws invader." Qhov kev pheej hmoo ntau ntawm kev pheej hmoo ntawm kev ua rau lub cev tsis muaj zog, ua kom ntev- lub sijhawm kho. Nyob rau hauv sib piv, ua ntej lawm version tau lees paub tias txawv teb chaws vim yog ib qho kev ua kom muaj kev tiv thaiv ntau ntxiv thiab yog li cuam tshuam rau kev ua tau zoo. Yog li no, qis immunogenicity yog ib qho ntawm somatropin lub ntsiab lus zoo.

** 2. Yog lub cev ua haujlwm simulation: **

Raws li ib qho zoo tshaj plaws replica ntawm ntuj HGH, Somatropin khi nrog lub siab sib luag ntawm lub siab thiab lwm yam kev sib tw ntawm cov teebmeem ntawm lub cev. Cov no suav nrog cov teebmeem ncaj qha thiab kev tsis ncaj ncees los ntawm kev txhawb nqa ntawm insulin {{1} Zoo li kev loj hlob ntawm kev loj hlob-1 (iGF-1) kev tsim khoom. Nws cov teebmeem ntawm lub cev muaj peev xwm ua tau zoo heev thiab muaj txiaj ntsig, feem ntau tshwm sim hauv: muaj zog txhawb cov khoom siv protein thiab kev loj hlob; Kev tswj cov ntxhia metabolism thiab kev txhim kho pob txha ceev; Kev txhawb nqa lipolysis thiab ua kom lub cev muaj roj faib; Thiab muaj kev cuam tshuam zoo tshaj plaws glucose metabolism, kev tiv thaiv kab mob, thiab lub siab noj qab haus huv.

** 3. Kev siv hluav taws xob dav: **

Vim nws cov haujlwm ntawm lub cev muaj zog, Somatropin muaj cov ntawv thov kev kho mob ntau ntau. Nws yog qhov kev hloov kho txheem kev kho rau kev loj hlob ib qho kev loj hlob (GHD) rau menyuam yaus, pab cov menyuam ua tiav kev loj hlob. Nws tseem siv los kho GUD hauv cov neeg laus, txhim kho lub cev muaj kev sib zog, muaj kev qoj ib ce, thiab lub neej zoo. Tsis tas li ntawd, nws qhov kev taw qhia tau nthuav dav kom suav nrog tus syner syndrome, thiab} {} {} {} {} {} {}} Hauv thaj tsam raug cai thiab kev tswj hwm, nws kuj tau siv los txhim kho kev rov qab ntawm cov neeg mob hlawv loj heev.

** 4. Nruj me ntsis tswj thiab xwm yeem: **

Raws li cov khoom synthesized los ntawm kev ua xyoob ntoo niaj hnub, Somatropin cov txheej txheem ntau lawm yog yuav ua rau tsis tshua muaj kev saib xyuas thiab tswj hwm kev nyab xeeb. Qhov no txhais tau tias txhua pob ntawm cov khoom muaj cov txiaj ntsig zoo, thiab cov neeg mob tau txais txiaj ntsig zoo, kom ntseeg tau kev kho mob tshwm sim los ntawm kev hloov pauv ntawm kev hloov pauv.

#### ** 4. Intuitive tsos thiab cov xim muaj xim **

Rau cov neeg siv kawg, Somatropin cov tsos yog nws cov kev txawj ntse tshaj plaws uas ntxim nyiam.

** 1. Lyophilized Hmoov Nrig: **

Nyob rau hauv ib lub suab nrov sib khi, tsis tau lees ib somatropin feem ntau tshwm sim yog xoob, dawb lossis tawm {{} dawb khov, tog twg los nyob rau hauv daim ntawv ntawm xoob lics lossis hmoov. Cov xim dawb no feem ntau yog vim yog qhov diffuse ntawm lub teeb los ntawm Lyophilized Hmoov. Raws li cov muaj protein ntshiab, Somatropin yog tsis muaj kob ntawm qib molecular. Cov khoom sib thooj xim yog qhov ntsuas ua ntej ntawm kev paug.

** 2. Kev daws teeb meem tom qab kho dua tshiab: **

Tom qab ua tiav kev rov ua tiav nrog cov kuab tshuaj txuam, ib qho tsis muaj kob, meej, thiab pob tshab nws yuav tsum tau txais qhov zoo. Txawm li cas los xij, vim muaj cov khoom ntawm cov protein ntawm cov protein thiab kev daws teeb meem tseem muaj kev sib cav sib ceg thiab cov xim tsis txaus ntseeg, tsis muaj teeb meem, nws yog feem ntau suav tias yog qhov ua tau zoo. Qhov kev ntsuas nruj, flocculent nag lossis txawv txawv ntawm cov xim sib txawv qhia tau tias muaj cov protein denaturation lossis paug thiab yuav tsum tsis siv kiag li.

#### ** Xaus **

Hauv cov ntsiab lus, Hgh Somatropin (amino acid 191) yog ntau tshaj li lub npe yooj yim ntawm lub npe. Nws yog qhov ua tau zoo tshaj plaws ntawm cov kev siv thev naus laus zis niaj hnub thiab tus qauv ntawm qhov sib sau ua ke ntawm cov qauv thiab kev ua haujlwm. Los ntawm nws meej 191-amino acid leadence rau nws cov peev txheej rhiab heev rau nws qhov kub thiab txias thiab pH rhiab heev; Los ntawm nws cov immunogenicity qis thiab zoo siab rau lub ntsej muag ntuj, rau cov kev daws teeb meem uas muaj txiaj ntsig zoo, muaj kev nyab xeeb, muaj ntau yam kev nyab xeeb. To taub nws cov yam ntxwv tsis sib haum tsis yog tswj kev nkag siab tshawb fawb tab sis kuj tseem yog qhov tseem ceeb rau nws cov chaw kuaj mob kom tsim nyog, chaw khaws khoom, thiab kev ntsuas kev nyab xeeb.