Lub hauv paus rau phau ntawv

MGF2

musclepic 5

Cov khoom lag luam

Tshooj 1 Kev faib tawm MGFS (los ntawm cov khoom lag luam thiab cov qauv hloov kho)

I. Luv e - peptide fragments (cov khoom siv hluavtaws ntau)

Cov khoom no feem ntau yog muab los ntawm cov khoom noj luv luv peptide (feem ntau kwv yees 24 -} cov amino acids nyob rau hauv ntev ntawm IGF-1C. Lawv muaj cov amino acid lead ua ke thiab raug hloov kho los ntawm cov hau kev xws li kev ua ntu zus los txhim kho kev ruaj ntseg thiab cov kev sib cuam tshuam ntawm cov khoom siv tau. Cov khoom lag luam no feem ntau pom hauv kev tshawb nrhiav chaw kuaj, siv rau hauv cov vitro ntawm kev sim thiab qee yam hauv Vivo qauv kev siv tau. Ntau lub suab hluavtaws peptide tuam ntxhab muab 24-25 AA cov qauv thiab qhia tawm cov ntsuas tsis xws li kev coj dawb huv thiab hnyav.

Ii. Tag nrho {{1 {1} ntev lossis ntev peptide cov ntawv (tag nrho- ntev ntev mgf / ikf-1ec analogs)

Tag nrho - ntev mgf lossis ze {}} ntev tshaj tawm thiab muaj kev sib ze heev rau cov khoom lag luam ntawm i ... 6}} 1ec. Cov ntawv qhia txog ntaub ntawv ntau yam kev ntsuas ntawm cov kev ntsuas hnyav tiav thiab ua ntu zus; Cov ntaub ntawv puv ntoob feem ntau siv rau kev tshawb fawb txog kev tshawb fawb txog kev tshawb fawb txog kev tshawb fawb txog kev tshawb fawb txog kev tshawb fawb txog kev tshawb fawb txog kev tshawb fawb txog kev tshawb fawb txog kev tshawb fawb txog ntau.

III. Cov tshuaj hloov kho thiab kho kom ruaj khov (piv txwv li, peg}} mgf, d} amino acid hloov, cyclation, thiab lwm yam)

Txhawm rau txhim kho hauv Vivo stability, polong ib nrab} lub neej, lossis txo cov amino acids, lossis ua lwm yam kev hloov kho tshuaj lom neeg. Peg - mgf yog ib qho ntev ntev- Lub neej ntawm kev ntsuas ib nrab (muaj peev xwm txo cov ntsiab lus ntev dua

IV. Fusion proteins thiab cov khoom xa tawm (txuas cov protein lossis txuas cov protein lossis encapsulation hauv nanoparticles / microparticts)

Hauv kev tshawb fawb thiab Bioengineering, MGF ua ntu zus kuj muaj cov protein, FC tawg, lossis txhawb nqa cov tsiaj ntawv ua tiav lossis ntau dua lub ntsej muag cov ntaub qhwv. Cov khoom no feem ntau- theem kuaj tau piav qhia hauv cov ntawv nyeem thiab cov qauv patent, nrog ob peb cov piv txwv ua haujlwm.

Tshooj 2 Cov Kev Ua Yeeb Yam thiab Muaj Peev Xwm Kev Muaj Peev Xwm (raws li cov ntawv sau thiab kev sim)

Mgf, raws li ib qho khoom lag luam ntawm iGF-1 lossis nws cov thooj tawm ntawm cov nqaij mos), cov leeg cov qia hlwb), thiab cuam tshuam nrog cell tsiv teb chaws thiab angiogenesis. Ntau yam nyob hauv cov vitro thiab cov tsiaj hauv kev tshawb fawb pom tias lub ntsej muag luv thiab txhim kho lawv cov kev sim ua ntej, yog li muab cov khoom siv sib luag, yog li muab cov khoom siv ntawm tes rau cov leeg. Txawm li cas los xij, reproppibility thiab kev tsis sib haum cov txiaj ntsig muaj; Qee qhov kev tshawb fawb ywj pheej tau ua tsis tiav qhov cuam tshuam tsis zoo ntawm MGF hauv qee cov qauv, qhia tias tseem muaj kev sib cav thiab tsis paub meej nyob hauv daim teb no. Kev txheeb xyuas thiab sim cov ntaub ntawv tham txog cov kab ke no.

Tshooj 3 physicochemical cov khoom (dav dav cov khoom, ntsuas ntsuas, thiab cov khoom sib txawv)

Amino Acid muaj pes tsawg leeg thiab hnyav hnyav

Tus txheeb ze lub cev hnyav ntawm lub cev qhuav dej / maj mam nyob nruab nrab ntawm cov ntaub ntawv sib txawv thiab cov khoom siv me me vim yog cov lus qhia acid). Protein - Zoo li cov khoom txhais tau tias "Tag nrho} ntev} ntev} ntev} ntev} (ze rau banding txawv ntawm igf-1ec). Cov nuj nqis tshwj xeeb yuav tsum yog raws li cov coa lossis cov ntaub ntawv loj rau cov ntaub ntawv muab los ntawm tus neeg xa khoom.

Solubility thiab ruaj khov: luv mgf peptides yog hydrophilic lossis tsis muaj zog poleptides, muaj raws li Lyophilized hmoov soluble hauv dej thiab cov kev daws teeb meem. Txawm li cas los xij, tus peptides 'kev ntxim nyiam kom muaj kev cuam tshuam thiab rhiab rau kev daws teeb meem, uas yog ib qho laj thawj uas muaj cov ntaub ntawv raw yog muab rau hauv lyophilized hmoov. PGylation lossis lwm yam kev hloov tshuaj lom neeg tuaj yeem hloov kho cov khoom siv dag zog ntawm peptides ntawm peptides thiab txhim kho lub sijhawm ruaj khov thiab voj voog.

Isoelectric taw tes thiab tshuaj lom neeg cov kev tawm tsam: lub e ntu ntawm mgf muaj ntau qhov yooj yim thiab acidic seem. Lub ISOELECTAL point thiab solubility nyob rau hauv cov pH txawv cuam tshuam rau nws cov qauv thiab cov txheej txheem analical. Kev pib ua ke thiab lwm qhov kev hloov kho, lossis lwm yam kev hloov kho cov molecular nto tsub nqi faib, yog li cuam tshuam nws cov kev ua kom huv thiab kev coj ua kom huv.

Cov tsos thiab cov xim (cov khoom siv raw): siab - Cov khoom siv zoo hluavtaws mgf feem ntau muaj nyob rau ntawm Lyophilized dawb lossis tawm {{kua txiv dawb. High - Kev pham Batches yog ntshiab dawb rau daus - dawb; Lub ntsej muag daj lossis xim av xim daj yuav qhia oxidation, seem ntawm cov kuab tshuaj, impurities, lossis qhov tsis raug cai. Tus neeg xa khoom daim ntawv pov thawj ntawm Kev Tso Cai (COA) yuav tsum hais cov tsos thiab cov lus qhia purity.

Tshooj 4 Tswjhwm Zoo thiab Cim (tsis-}}} Cov tswv yim piav qhia): Siab} Zoo mgf yuav tsum muaj ua tiav zoo

Cov ntaub ntawv, suav nrog tab sis tsis txwv rau: HPLC Purity Spectra, Maldi {} miece}} kev pom zoo ntawm molecen Tshawb nrhiav thiab cov koom haum tswj hwm feem ntau pom zoo kom siv siab- Daws teeb meem nrog HPLC kom paub meej tias cov khoom xa tawm Isotopic thiab Purity Peak zoo. Tej kev ua lag luam / muab kev lag luam yuav tsum muaj cov khoom sib xyaw ua ke thiab cov lej. Ntau txoj kev ntsuas tshuaj rau kev txhim kho peptide stabilization kuj tseem tau piav qhia hauv cov ntaub ntawv Patent thiab cov qauv txheej txheem.

Tshooj 5 Daim Ntawv Thov Qhia Txog Cov Lus Qhia (Kev Tshawb Fawb Siv thiab Kev Lag Luam Tej Peev Xwm)

I. Kev tshawb fawb thiab kev siv tshuaj rau Vitro thiab kev raug mob Vivo, kev kho mob lub tshuab raj ntawm cov leeg nqaij

Ii. Tsim kev tsim kho: Pegylation thiab Fusion Protein cov tswv yim muab cov lus qhia rau Long} Lub Sij Hawm Tsim Kho, Ua kom txhim kho cov tshuaj papulation kev tsim kho thiab txhim kho kev kho mob siv tau.

III. Cov tshuaj kho mob thiab cov ntaub so ntswg muaj peev xwm siv tau ntawm cov nqaij mos ntawm cov qia cell hloov pauv, thiab kev ua haujlwm ntawm Biomaterials / Scaffolds. Nws yog ib qho tseem ceeb kom qhia txog tias ntau daim ntawv thov tseem nyob hauv cov theem kev sim lossis xwm txheej.

TSHOOJ 6 Kev Nyab Xeeb, Muaj Kev Sib cav, thiab Kev Txiav Txim Siab

Tam sim no, tsis muaj kev pom zoo hauv zej zog kev kawm txog kev ua tau zoo thiab kev nyab xeeb ntawm MgF. Qee cov ntaub ntawv tshawb fawb txhawb nqa nws cov proliferative thiab kho cov peev xwm, thaum lwm tus tsis tuaj yeem kho cov txiaj ntsig no lossis tau qhia kev ua tau zoo. Tsis tas li ntawd xwb, vim MGF cuam tshuam nrog iGF {}} 1 system, nws lub peev xwm los txhawb kev txhawj xeeb ntawm tes, feem ntau hauv cov neeg muaj ntau yam, xav tau kev saib xyuas zoo. Qee cov khoom uas tsis muaj kev txwv ntawm kev ua lag luam tau txawm pom muaj cov khoom xyaw lossis cov teeb meem purity uas tsis phim daim ntawv lo. Yog li ntawd, kev tshaj tawm thiab kev siv yuav tsum ua raws li txoj cai hauv zos, cov cai hauv zos thiab kev coj ua zoo. Cov chaw ua si los tiv thaiv doping kuj tseem xav txog qee cov tshuaj muaj peev xwm siv tau cov cai kev sib tw thiab kev sim siab thaum siv. Txheeb xyuas cov ntawv tseem taw qhia kev tsis ncaj ncees thiab cov teeb meem reproppility nyob rau hauv daim teb no, xav cov kws tshawb fawb yuav tsum muaj kev cuam tshuam nyuaj rau kev sim thiab kev siv tau ywj pheej.

Tshooj 7 cia, tuav

Thiab kev thauj mus los (cov lus qhia ua kom raws li cov ntaub ntawv siab thiab khaws cia rau hauv lyophilized thiab khaws cia} {- kub ib puag ncig. Cov chaw muag khoom yuav muab cov sijhawm cia thiab cov lus pom zoo hauv lawv cov tog tog CO lossis MSDS. Kev thauj mus los yuav tsum ua raws li cov kev cai cuam tshuam rau cov tshuaj reagents lossis tshuaj lom neeg. Cov ntaub ntawv xov xwm thiab daim ntawv pov thawj tshuaj yuav tsum tau tshuaj xyuas cov zaub mov xa tuaj kom ntseeg tau tias muaj kev ntxhov siab. Kev tuav tau pom zoo kom nqa tawm hauv ib qho chaw uas tau raws li kev kuaj cov qauv kev nyab xeeb thiab ua raws li kev tswj hwm kev ua haujlwm ntawm kev ua haujlwm kev tiv thaiv thiab khib nyiab.

Tshooj 8 Cov Lus Qhia Tau Txais Cov Lus Taw Qhia thiab Cov Ntsiab Lus Tseem Ceeb rau kev txheeb xyuas high- cov khoom lag luam zoo (kev ua tiav)

High - Cov ntaub ntawv zoo MGF yuav tsum tau txais cov cim tshwj xeeb hauv qab no: Ua kom tiav cov ntaub ntawv teev tseg (COA) Verifying third-party testing or peer-reviewed research citations before purchasing, and inquiring about the ethical and regulatory compliance of the product's synthesis methods (to avoid unlicensed human-grade production chains), are all necessary steps to reduce risk. Tsis txhob cia siab rau cov tsis tau tso tseg "kev lees paub ua tau zoo" lossis tsis qhia npe tshwj xeeb.

TSHOOJ 9 TIBXUS: Kev ua tim khawv thiab cov pov thawj ua ntej

Raws li kev nthuav tshwj xeeb sib txawv ntawm lub iGF {{}} 1 tsev neeg, mgf's luv peptide tau qhia muaj peev xwm ua tau muaj peev xwm hauv cov kev tshawb fawb thiab kev rov ua haujlwm ntawm cov kev tshawb fawb. Txawm li cas los xij, kev kho mob thiab loj {{{3} Kev lag luam teev tseem xav tau ntau dua} Zoo tshaj plaws, kev ntsuas kev nyab xeeb. Rau cov chaw tshawb nrhiav thiab cov qauv tsim tawm, xaiv cov khoom lag luam thiab cov pov thawj nrog cov qauv zoo, thiab kev cai lij choj, thiab kev cai yog qhov ua ntej ntawm daim teb no. Rau cov neeg nyeem dav dav lossis tsis - Cov chaw kuaj mob thiab kev sim ua kom tsis muaj kev cia siab rau tib neeg yog lub luag haujlwm ntawm kev noj qab haus huv thiab kev nyab xeeb rau pej xeem. Yog xav paub cov ntaub ntawv ntxiv qub ntawm cov roj mgecular biology keeb kwm yav dhau los ntawm mgf, tus sawv cev sib txawv peptide, thov saib cov ntawv sau sib txawv thiab cov khoom lag luam kev tshwj xeeb.