Taw qhia

FOXO42

Factory 2

musclepic 4

real order

Khoom Description

1. Kev faib tawm thiab cov yam ntxwv zoo

Los ntawm kev tsim qauv thiab kev pom, FOXO4 peptides tuaj yeem muab faib ua pawg hauv qab no:

Thawj qeb yog linear L-type FOXO4 peptides, tsim los ntawm ntuj L-hom amino acids, nrog cov qauv ze rau cov khoom qub protein. Cov no feem ntau yog siv rau kev tshawb fawb mechanism thiab hauv vitro thwmsim.

Cov qeb thib ob yog FOXO4-DRI peptides, piv txwv li, D-hom thim rov qab- xaj peptides, uas yog tam sim no feem ntau tau kawm thiab siv qeb. Lawv cov amino acid sequence yog thim rov qab, thiab lawv tag nrho tsim los ntawm D-hom amino acids, txhim kho kev ruaj ntseg thaum tswj kev sib raug zoo ntawm qhov chaw.

Cov qeb thib peb yog hloov kho FOXO4 peptides, suav nrog terminal acetylation, amidation, lossis kev sib txuas rau ntawm tes -peptide tawg, siv los txhim kho cellular uptake efficiency thiab hauv vitro thiab vivo stability.

2. Cov txiaj ntsig tseem ceeb thiab kev tshawb fawb tus nqi

Qhov zoo tshaj plaws ntawm FOXO4 peptides nyob rau hauv lawv lub hom phiaj siab heev thiab molecular mechanism ntawm kev ua. Los ntawm kev cuam tshuam tshwj xeeb hauv kev sib cuam tshuam ntawm FOXO4 thiab p53, peptide no nthuav tawm cov kev xaiv zoo hauv cov qauv kev laus ntawm tes.

Thib ob, FOXO4-DRI yog tsim los nrog D-amino acids, muab nws muaj zog tiv thaiv proteases thiab txo qis degradation tus nqi hauv cov ntshav los yog kab lis kev cai, ua rau muaj kev sim ntau dua.

Thib peb, hom peptide no muaj qhov hnyav molecular hnyav, cov qauv ntshiab, yooj yim rau kev sib txuas thiab ua kom huv, thiab nthuav tawm cov khoom zoo - rau-batch sib xws, ua rau nws haum rau ntev - siv los ntawm cov chaw tshawb fawb thiab cov tuam txhab biotechnology.

Tsis tas li ntawd, FOXO4 peptides tsis ncaj qha koom nrog hauv kev kho cov noob caj noob ces lossis kev cuam tshuam tsis tuaj yeem, tab sis tsom mus rau kev tswj hwm ntawm theem kev taw qhia, muab kev nyab xeeb zoo thiab kev tswj hwm hauv kev tshawb fawb yooj yim.

3. Physicochemical Properties

FOXO4 peptides yog nruab nrab-ntev ua haujlwm polypeptides, feem ntau nrog ib qho hnyav molecular nyob rau hauv 3-5 kDa ntau yam, nyob ntawm seb qhov sib lawv liag thiab kev hloov kho txoj kev.

Lub cev, FOXO4 raw cov ntaub ntawv feem ntau muaj nyob rau hauv lyophilized hmoov daim ntawv, uas yog structurally ruaj khov thiab yooj yim rau khaws cia rau lub sij hawm ntev.

Hais txog solubility, peptide no feem ntau yog soluble hauv dej deionized, PBS, lossis tsis muaj zog acidic buffers. Cov khoom uas muaj ntau dua ntawm hydrophobic sequences yuav tsum tau maj mam sonication los yog qeeb vortexing thaum pib dissolution.

Hais txog kev ruaj ntseg tshuaj, D-hom FOXO4-DRI peptides pom zoo rau qhov kub thiab txias, pH hloov pauv, thiab enzymatic degradation dua li L-hom peptides, ua rau lawv tsim nyog rau kev sim ua haujlwm nyuaj.

4. Xim thiab tsos yam ntxwv

High-purity FOXO4 peptides feem ntau yog dawb los yog tawm -cov hmoov dawb lyophilized nrog xoob, zoo nkauj zoo nkauj thiab tsis pom impurities.

Yog tias cov khoom zoo li daj ntseg daj, nws feem ntau cuam tshuam nrog peptide saw ntev, amino acid muaj pes tsawg leeg, lossis cov txheej txheem lyophilization thiab feem ntau tsis cuam tshuam rau nws txoj kev tshawb fawb.

High- cov khoom lag luam zoo yuav tsum muaj cov kev daws teeb meem meej thiab pob tshab tom qab rov tsim dua tshiab, tsis muaj nag lossis daus los yog cov teeb meem flocculent. Qhov no kuj yog ib qho tseem ceeb ntawm cov tsos mob rau kev txiav txim siab purity thiab cia tej yam kev mob.

5. Cov txheej txheem ntau lawm thiab Purity Control

FOXO4 peptides feem ntau yog npaj siv cov khoom siv-theem peptide synthesis thev naus laus zis, tsim kom tiav peptide chains los ntawm stepwise condensation.

Tom qab kev sib xyaw ua ke, ntau cov kauj ruam ntawm kev tiv thaiv, lysis, thiab thim rov qab - theem siab - ua kua chromatography purification yog yuav tsum tau thaum kawg tau siab - purity raw khoom.

Kev tshawb fawb-qib siab- zoo FOXO4 peptides feem ntau muaj purity ntau dua 95%, nrog siab - cov ntawv thov kev tshawb fawb kawg ncav cuag 98% lossis siab dua.

Hauv kev tswj kom zoo, qhov hnyav ntawm molecular, qhov raug raws li qib, thiab qib impurity feem ntau tau lees paub siv HPLC, huab hwm coj spectrometry, thiab tshuaj xyuas cov amino acid muaj pes tsawg leeg.

6. Daim Ntawv Qhia Kev Qhia thiab Kev Tshawb Fawb

FOXO4 peptides feem ntau yog siv rau hauv cov kev tshawb fawb theem pib xws li kev tshawb fawb ntawm cellular aging mechanism, p53 signaling pathway analysis, thiab validation of senescent cell model.

Hauv kev sim hauv vitro, nws feem ntau siv los txheeb xyuas qhov kev coj cwj pwm lom neeg ntawm cov hlwb senescent los ntawm cov hlwb ib txwm muaj.

Nyob rau theem kev sim tsiaj, FOXO4-DRI peptides yog siv los tshawb nrhiav cov kev tswj hwm txoj hauv kev muaj feem cuam tshuam txog kev laus, tab sis qhov no tseem txwv nruj rau theem tshawb fawb thiab tshawb nrhiav.

Tam sim no, peptide no tsis yog cov khoom siv raw rau cov tshuaj kho mob niaj hnub lossis cov khoom noj qab haus huv; nws tus nqi yog tsuas yog xav txog nyob rau hauv kev tshawb fawb innovation thiab theoretical validation.

7. Cov lus pom zoo khaws cia thiab thauj mus los

FOXO4 peptide raw cov ntaub ntawv raug pom zoo kom khaws cia ntawm -20℃lossis qis dua, tiv thaiv lub teeb. Rau kev siv sijhawm luv, nws tuaj yeem khaws cia ntawm 2-8 degree.

Cov hmoov lyophilized pom zoo stability; tom qab reconstitution, nws yuav tsum tau aliquoted thiab khaws cia kom tsis txhob rov khov -thaw cycles.

Thaum lub sij hawm kev thauj mus los, qhov qis - qhov kub thiab txias, ib puag ncig qhuav yuav tsum tau tswj xyuas kom ntseeg tau tias peptide saw kev ncaj ncees thiab cov khoom sib xws.

8. Cov ntsiab lus

Zuag qhia tag nrho, FOXO4 peptides yog ib chav kawm ntawm kev ua haujlwm polypeptide raw cov ntaub ntawv nrog cov qauv meej, zoo - cov txheej txheem tau hais tseg, thiab cov txiaj ntsig tshawb fawb tseem ceeb. Sawv cev los ntawm FOXO4-DRI, lawv tuav txoj haujlwm tseem ceeb hauv kev tshawb fawb txog kev laus ntawm tes los ntawm kev tsim tshwj xeeb molecular.

Raws li lub hauv paus ntawm kev ua kom muaj purity siab, tus qauv cia, thiab kev sim tsim tsim nyog, FOXO4 peptides muab cov cuab yeej txhim khu kev qha thiab txiav -ntev cov cuab yeej rau kev tshawb fawb hauv lub neej thiab kev tshawb fawb molecular mechanism, thiab tseem nteg cov khoom siv hauv paus rau yav tom ntej cov lus qhia txog kev tshawb fawb.