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Cov khoom lag luam
#### ** 1. Txheej txheem cej luam ntawm follistatin 344 peptide **
Follistatin 344 (FS344) yog ib qho glycoprein muaj li 344 amino acids thiab belongs rau Follistatin tsev neeg. Nws cov tub ntxhais muaj nuj nqi yog txhawm rau tswj cov kev muaj peev xwm ntawm tes, kev sib txawv thiab cov leeg nqaij cuam tshuam nrog kev hloov pauv thiab inhibiting cov tswv cuab ntawm kev hloov pauv kev loj hlob- (xws li illfamily (xws li attadin thiab myostatin). FS344 tau nyiam ntau tus neeg nyob hauv cov kab mob biomedical, tshwj xeeb tshaj yog nyob rau hauv kev kho cov kab mob ntawm cov kab mob thiab cov tshuaj tswj me me.
#### ** 2. Cov txheej txheem kev faib tawm ntawm Follistatin 344 **
Raws li cov qauv hloov kho, muaj kev sib txawv ntawm cov qauv sib txawv thiab cov qauv formulation thiab FS344 tuaj yeem faib ua cov hauv qab no:
##### ** 1. Ntuj tsis paub meej follistatin 344 **
- ** Tus yam ntxwv tsim qauv **:
Nws muaj 344 cov amino acids thiab muaj 3 tus thawj coj ua haujlwm:
- ** N-terminal domain ** (1-63): koom nrog kev ua haujlwm khi.
- ** CENTRAL DIM ** ({64-215}}): nplua nuj nyob rau hauv cysteine, ruaj khov txoj cai peb-seem.
- ** C-Terminal Domain ** (216-344): Txhim kho qhov kev sib cuam tshuam nrog cov proteoglycans.
- ** ** Zoo **:
- High affinity binding rau orgin thiab myostatin.
- Lub ntuj hloov tau khaws cia ua tiav kev ua haujlwm roj ntsha.
- ** Lub cev thiab tshuaj lom neeg **:
- Molecular hnyav: hais txog 38-40 kda (suav nrog kev hloov kho glycosylation).
- isoelectric taw (PI): 5. 2-5. 8.
- Solubility: yooj yim soluble hauv nruab nrab tsis (xws li PBS).
- Xim thiab morphology: Lyophilized hmoov yog dawb xoob xoob, thiab kev daws teeb meem yog xim tsis muaj kob.
##### ** 2. Acetylated Follistatin 344 **
- ** Kev hloov kho txheej txheem ":
N-terminal lossis cov lysine seem yog acetylated los txhim kho kom tsis kam rau enzymatic hydrolysis.
- ** ** Zoo **:
- Lub neej ntev ib nrab lub neej hauv vivo (los ntawm ob peb teev mus rau {2}} teev).
- Txo cov impunogenicity.
- ** HYMICOCHEMICAL YAM **:
- Lub cev hnyav nce ntxiv los ntawm kwv yees li 42 teev / acetylation site.
- Solubility: Yuav tsum tau yaj nyob rau hauv ib tug ntas muaj ib qho me me ntawm cov organic huab cua (xws li 5% dmo).
- Xim: Lyophilized hmoov dawb dawb rau cov hmoov dawb.
##### ** 3. Pegylated Follistatin 344 **
- ** Kev hloov kho txheej txheem ":
Polyethylene glycol (peg) molecules (feem ntau {20-40 kda) tau txuas los ntawm kev sib txuas lus.
- ** ** Zoo **:
- Tseem ntev ntev ntev ntev-lub neej (mus txog ntau dua 72 teev).
- Txhim kho kev siv tshuaj ua haujlwm tau zoo thiab txo kev tshem tawm lub raum.
- ** Lub cev thiab tshuaj lom neeg **:
- Molecular hnyav: {{{60-80 kda (nyob ntawm qhov ntev ntawm PG saw).
- Solubility: dej-dej-doluble, tab sis tuaj yeem tsim cov tshuaj colloidal.
- Xim: Kev daws teeb meem me ntsis opalescent, thiab lub lyophilized hmoov yog cov khoom qab dawb.
##### ** 4. Truncated follistatin 344 (FS288 / FS315) **
- ** Cov qauv nta **:
Qee cov amino acids ntawm C-Terminus tau ploj lawm (xws li FS288 muaj tsuas yog 288 amino acids).
- ** ** Zoo **:
- Lub cev me me molecular hnyav (txog 32 kda), txhim kho cov ntaub so ntswg sib cais.
- khaws cov attains kev ua kom muaj peev xwm, tab sis yuav txo qis kev ruaj khov.
- ** HYMICOCHEMICAL YAM **:
- isoelectric point yog ze rau haiv neeg FS344 (PI 5. 0-5. 5).
- Solubility: Zam kev haus ntsev ntau kom tiv thaiv kev sib sau ua ke.
#### ** 3. Cov ncauj lus kom ntxaws ntawm lub tshuab physicochemical **
##### ** 1. Thermal Stability **
- Unmodified FS344 yog qhov ruaj khov ntawm 4 degree rau 1-2 lub lis piam thiab tuaj yeem khaws cia ntawm {}} degree rau ntau tshaj 6 lub hlis.
- Pegylated FS344 yog ntau dua cov cua sov-tiv taus vim muaj kev tiv thaiv PEG PEG thiab tuaj yeem tiv thaiv chav sov ntev.
##### ** 2. Acid-puag stability **
- Qhov ntau heev ntawm PHIG: 6. 5-7. 5.
- heev pH (<4 or >9) ua rau tsis muaj kev cuam tshuam tsis pub dhau los, tsim cov turbidity lossis nag lossis daus.
##### ** 3. Cov yam ntxwv spectral **
- Uv nqus lub ncov: 280 nm (Tryptophan / Tyrosine).
- Fluorescence Cov yam ntxwv: kev zam wavitation 280 nm, emission wavelength 340 nm (rau endorogenous fluorescence Nrhiav tau).
#### ** 4. Daim ntawv thov zoo ntawm ntau hom follistatin 344 **
| ** Qeb **|** Cost Zoo **|** Thaj chaw thov ** |
|-------------------------|---------------------------------------|-----------------------------|
| Ntuj unmodified hom|HIEL bi bi activity activity activity,,,,, Tsawg Immunogenicity|Kev Tshawb Fawb Kev Tshawb Fawb Cov Khoom Siv, hauv Vitro Cell sim |
| Acetylated hom|Txuas ib nrab-lub neej, txo tus nqi|Cov Qauv Tsiaj, Kev Tshawb Fawb Kab Mob Kab Mob |
| Pegylated Hom|Lub sijhawm ntev nrhiav tau, txhim kho cov kws muag tshuaj papharminin|Kho Mob Sims, Tshuaj Tenstrophy Kev Kho Mob |
| Nruas hom|Siab cov ntaub so ntswg permeability|Cov thawj coj hauv zos (xws li kev txhaj tshuaj inframuscular) |
#### ** 5. Kev sib raug zoo ntawm cov xim thiab tsim daim ntawv **
1. ** Labophilized hmoov **: Dawb rau off-dawb, nyob ntawm yam ntxwv purity thiab hloov kho (popylation tej zaum yuav flocculent).
2. ** Lub xeev daws teeb meem **:
- Yam tsis muaj qauv: tsis muaj xim thiab pob tshab.
- Pegylated hom: me ntsis opalescent (colloidal cov khoom).
3. ** Lub xeev denatured **: Turbid lossis daj (qhia txog kev ua kom tsis pom zoo lossis oxidation).
#### ** 6. Kev Nyab Xeeb thiab Kev Lom Zem **
- ** Immunogenicity **: Hom kev tsis paub tsis meej yuav ua rau cov tshuaj tiv thaiv theem qis, uas yog txo ntxiv ntxiv tom qab pgylation.
- **Acute toxicity**: LD50 >100 mg / kg hauv kev sim tsiaj (kev txhaj tshuaj tiv thaiv hauv cov nas).
- ** OU-TIM TIM NYOG **: Cov koob tshuaj lom zem ntev yuav cuam tshuam nrog txoj kev taug mus rau txoj kev tgf-signaling, thiab nplooj siab thiab raum ua haujlwm yuav tsum tau saib xyuas.
#### ** 7. Cov Lus Qhia Yav Tom Ntej **
1. ** Kev hloov kho tshiab tshiab **: xws li fatty acid charmov (palmitoylation) los txhim kho cov membrane nkag mus.
2. ** Lub kaw lus xa khoom **: Nanoparticles encapsulate FS344 txhawm rau txhim kho cov leeg nqaij tshwj xeeb.
3. ** Kev khomial hloov pauv **: Theem III Kev sim rau Duchenne Tawv (DMD) thiab muaj hnub nyoog ntsig txog cov leeg leeg.
#### ** 8. Ntsiab lus **
Qhov ntau-qeb sib txawv ntawm follistatin 344 tau raws li cov kev xav tau sib txawv los ntawm kev tshawb fawb yooj yim rau kev kho mob. Cov kev cai nplua ntawm nws lub cev nqaij daim tawv thiab tshuaj lom neeg (xws li pegylation thiab acetylation) ntau txhim kho cov xim thiab morphology muab qhov ntsuas pom tseeb rau kev tswj kom zoo. Nyob rau yav tom ntej, nrog kev nrhav ntawm kev hloov kho tshuab thev naus laus zis, FS344 yuav tsum tau ua tiav cov teb ntawm cov tshuaj kho mob thiab kev tiv thaiv kev laus.
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