Hauj lwm lawm

Kev tsis

Lub hauv paus rau phau ntawv

peptides

product-1400-1400

Cov khoom lag luam

### ace -031 peptide: kev ntsuam xyuas lub cev

Ace -031 (Actionin recertor hom iib fusion protein) yog qhov rov qab tsim protein) yog kev tswj hwm cov leeg kev loj hlob thiab cov metabolism. Nws cov kev ua tub ntxhais lub tshuab yog los txhawb cov leeg pob txha hypertrophy thiab inhibit leeg kev sib txuam - (tgf-) superfamily signaling txoj kev (xws li illosin thiab myostatin). Kab lus no yuav siv cov hoob dis aus, physichochemical cov khoom, cov txiaj ntsig zoo thiab cov ntawv thov ntawm ACE {3}}.

#Khaum 1. Cov qauv thiab kev siv tshuab ntawm Ace -031

Ace -031 yog tsim ntawm cov neeg sib cav sib ceg Cov qauv no muab nws cov yam ntxwv hauv qab no:

1. ** High affinity khi **: The Actriib sau tau tshwj xeeb heev khi rau tgf- tsev neeg ligands (xws li willinin a, myostatin).

2. ** Txuas ntxiv ib nrab-lub neej **: IGG FC tawg tau ntau lub neej nyob rau hauv Vivo los ntawm kev sib tw ua haujlwm los ntawm Neonatal FC recotriated los ntawm Neonatal FC recotriated los ntawm neonatal fc reciator (FCRN).

3. **Signal pathway inhibition**: By competitively binding to ligands, blocking their interaction with cell membrane receptors, inhibiting SMAD2/3 signaling, and thus promoting muscle anabolism.

### 2. Kev faib tawm thiab derivatives ntawm ace -031

Raws li cov qauv hloov kho thiab ua kom zoo dua qub, ace -031}} thiab nws cov analogs tuaj yeem faib ua cov hauv qab no:

#### ** 1. Basic ace -031} **

- ** QAUV **: Daim Qauv Fusion Protein, Muaj Actriib Extractacellular sau thiab tib neeg IGG1 FC ntu.

- ** ** Zoo **:

- Kev ua haujlwm zoo heev: Nws tuaj yeem nce cov leeg leeg rog thiab lub zog hauv cov tsiaj tsiaj.

- Tsawg immunogenicity: tib neeg tsim qauv txo qhov kev pheej hmoo ntawm cov tshuaj tiv thaiv antibody.

- ** Lub cev thiab tshuaj lom neeg **:

- Molecular hnyav: li 110 kda (55 kda hauv monomicic daim ntawv, feem ntau muaj nyob ua siters).

- Solubility: soluble hauv nruab nrab tsis (xws li PBS), nrog kev pom zoo ntawm pH 7.4.

- Xim: Lyophilized hmoov dawb lossis tawv-dawb, thiab ua kua dawb ua kua tom qab rov kho dua.

- ** Cov ntaub ntawv tshawb fawb tau siv tau siv los kho Duchenne leeg dystrophy (dmd) thiab Cancer Cachexia.

#### ** 2. Pegylated ace -031} **

- ** QAUV **: Polyethylene Glycol (Peg) cov saw) txuas mus rau cov hom hauv paus kom txhim kho cov tshuaj tiv thaiv kab mob.

- ** ** Zoo **:

- Txuas ntxiv ib nrab-lub neej: Pegylation txo cov kev tshem tawm lub neej thiab txuas ntxiv lub neej ib nrab-lub neej rau {3}} hnub.

- Txo cov koob tshuaj zaus: kev txhaj tshuaj subcutaneous ib zaug ib lub lim tiam muaj peev xwm tswj tau cov txiaj ntsig zoo.

- ** HYMICOCHEMICAL YAM **:

- Molecular hnyav: sib txawv nrog peg saw ntev (feem ntau nce los ntawm 20-40 kda).

- solubility: kev kawm qib siab ntawm pevylation yuav tsum tau ua kom zoo rau kev zam kev sib sau ua ke.

- Xim: Lyophilized hmoov yuav tau zoo me ntsis daj vim yog hom PEG, thiab kev daws tau cov tshuaj tseem meej.

#### ** 3. Mutant variants (xws li ace -031- v1) **

- ** QAUV **: Txhim kho qhov ruaj khov thiab kev ua si los ntawm kev hloov pauv kis (xws li n-txuas glycosylation site kho).

- ** ** Zoo **:

- Txhim kho thermal stability: tom qab kev sib hloov, nws tuaj yeem khaws cia ntawm 4 degree rau lub sijhawm ntev dua, txo cov kev hloov pauv ntau dua, kev ua haujlwm dua.

- Txhim kho ntau lub potency: qhov khi kev sib txawv ntawm qee cov variants yog nce los ntawm {1} {zaus.

- ** Lub cev thiab tshuaj lom neeg **:

- isoelectric taw (PI): nws yuav hloov los ntawm 7.8 txog 8.2 vim kev hloov pauv.

- Xim: zoo ib yam nrog cov hom yooj yim, dawb Lyophilized hmoov.

#### ** 4. Peptide Fragment Gimics **

- ** QAUV **: MOLECLE MOLECLE PEPTATE PEPECTION tau tsim los ntawm cov actriib khi cov npe (txog {{1} cov amino acids).

- ** ** Zoo **:

- Qhov ncauj muaj peev xwm: qee qhov ua tau peptides tuaj yeem tiv thaiv kev ua haujlwm tsis txaus ntseeg.

- Tus nqi tsawg: Tshuajntsuam cov tshuajntsib hloov cov lus qhia tseem ceeb dua.

- ** Lub cev thiab tshuaj lom neeg **:

- Molecular hnyav: 1. 5-3. 5 kda.

- Solubility: qee qhov hydrophobic peptides xav tau DMO los pab yaj.

- Xim: Feem ntau cov hmoov dawb paug, qee tus peptides uas muaj cov amino acids uas muaj lub teeb daj.

### 3. Cov ntsiab lus zoo ntawm ace -031

1. ** Hom phiaj tshwj xeeb **: precise inhibition ntawm myostatin), zam kev phiv los ntawm kev tawm tsam dav-tsis sib haum.

2. ** Kev ua tau zoo tseem ceeb **: Hauv cov tsiaj tsiaj, cov leeg nqaij yuav tau nce ntxiv los ntawm {1}}%.

3. ** Qhia txog kev qhia dav dav ": Npog cov mineed ib yam (xws li DMD), muaj hnub nyoog ntsig txog muaj hnub nyoog, muaj hnub nyoog Cachexia, thiab lwm yam.

4. ** Kev tsim kom tau zoo **: tuaj yeem tsim mus rau Lyophilized hmoov txhaj, prefilled koob txhaj tshuaj lossis ntev-ua haujlwm-tso cov nqi.

### 4. Hauv kev ntsuas ntawm lub cev thiab tshuaj lom neeg

1. ** STABLY **:

- Thermal stability: kev ua ntawm hom theem pib txo qis los ntawm<10% within 72 hours at 25°C, and the mutant can tolerate 37°C for 48 hours.

- Photosensitivity: Khov-qhuav-qhuav yuav tsum tau muab cia ntawm lub teeb, raws li lub teeb yuav ua rau oxidation ntawm FC ntu.

2. ** Cov yam ntxwv spectratics **:

- Uv nqus lub ncov: 280 nm (feem ntau yog los ntawm tryptophan thiab Tyrosine).

- Fluorescence Cov yam ntxwv: Kev zam Wavitach 280 nm, emission Peak 340 NM (rau kev paub huv si tau pom).

3. ** Cov hau kev ntsuas "**:

- SDS-nplooj ntawv: Tshawb xyuas Dimer ncaj ncees (kwv yees 110 KDA Band).

- Cov Khoom Siv Loj (LC-MS): Paub meej molecular hnyav thiab glycosylation hloov kho.

- Circular dichroism (CD): monitor secondary structure (β-folding ratio> 60%).

### 5. Cov ntawv thov kev kho mob thiab cov kev sib tw

#### ** 1. Soj ntsuam xyuas kev nce qib **

- ** DMD theem II kev sim ** (NCT01099761):

- Cov txiaj ntsig: 6 lub hlis ntawm kev kho mob nce cov nqaij ntshiv hnyav los ntawm 1.2 kg (vs 0. 3 kg hauv pawg placebo).

- Vim li cas rau kev txiav tawm: nce kev pheej hmoo ntawm los ntshav (cuam tshuam nrog Angiogenesis Inhibition).

- ** ** ** Cancer Cachexia **: Tsis tau sau cov theem III vim muaj kev ua tau zoo tsis txaus.

#### ** 2. Cov kev sib tw loj **

- ** Kev nyab xeeb nyab xeeb yog dav koom nrog kev kho cov nqaij mos mos, uas yuav ua rau muaj kev phiv tshuaj fibrosis lossis cov mob plawv.

- ** Tus nqi them ** **: Cov roj ntsha protein ntau lawm yog kim (txog $ 5000 / g).

- ** Kev sib tw los ntawm lwm txoj kev kho mob **: xws li monoclonal Antibodies poostatin (stamulumab) lossis kev kho cov gene.

### 6. Yav Tom Ntej Kev Loj Hlob

1. ** Cov ntaub so ntswg tshwj xeeb **: Siv nanoparticles rau phiaj leeg leeg thiab txo cov kab ke.

2. ** Bifional Fusion Protein **: Ua ke nrog IGF -1}} Cov}} Cov neeg} Tshaj tawm cov leeg nqaij rov ua dua tshiab.

3. ** Kev tshawb xyuas qhov ncauj ntawm qhov ncauj **: Kev tshawb fawb txog kev nkag mus rau kev sib tw ua raws li peptide mimetics.

### xaus

ACE -031, raws li ib tug sawv cev ntawm cov leeg nqaij me me, tau ua kom pom lub peev xwm kho cov leeg nqaij tsis muaj kab mob. Txawm hais tias kev hloov kho hauv chaw kho mob ntsib kev cov nyom, nws xav kom ua tiav cov ntawv thov tawg hauv kaum xyoo tom ntej ntawm kev ua haujlwm zoo los ntawm kev ua haujlwm zoo thiab cov thev naus laus zis. Cov kws tshawb nrhiav yuav tsum tau pom qhov sib npaug ntawm kev ua tau zoo, kev nyab xeeb thiab them nyiaj rau cov kev kho mob no kom tau txais txiaj ntsig zoo.