Hauj lwm lawm

Kev tsis

Taw qhia

Epitalon2

musclepic 6

Khoom Description

1. Cov ntsiab lus tseem ceeb thiab keeb kwm yav dhau

Epitalon tau pib tsim los ntawm pab pawg tshawb fawb Lavxias thaum kawm txog kev hloov pauv hauv lub caj pas pineal. Nws tau siv los ua cov peptide ntuj Epithalamin muab rho tawm los ntawm cov tsiaj pineal caj pas. Vim muaj cov khoom siv sib txawv thiab tsis ruaj khov ntawm cov khoom siv ntuj tsim, cov khoom siv peptides zoo li Epitalon muab kev ntseeg siab, tsim qauv, thiab cov qauv zoo - txhais lwm txoj hauv kev.

Nws cov qauv yog yooj yim heev, muaj tsuas yog plaub amino acids. Qhov tsawg kawg ib ntus muab nws zoo heev stability, solubility, thiab yooj yim ntawm tuav. Raws li kev tshawb fawb peptide luv luv, Epitalon muaj tus nqi siv nyob rau hauv molecular biology, kev tshawb fawb ntawm cov txheej txheem kev laus, thiab kev tshawb fawb ntawm cell signaling pathways.

2. Kev faib tawm (raws li daim ntawv thov thiab cov qauv zoo)

Common Epitalon raw khoom hmoov nyob rau hauv kev ua lag luam tuaj yeem muab faib ua pawg hauv qab no raws li lawv daim ntawv thov thiab qib zoo:

1. Pharmaceutical Grade (High Purity Grade)

Feem ntau, lub purity yog ntau dua los yog sib npaug rau 98%, nrog nruj tswj impurities. Tsim nyog rau qib siab - kev sim, hauv - kev tshawb fawb molecular tob, thiab kev tsim qauv.

Nta:

Txheeb xyuas los ntawm ntau txoj hauv kev suav nrog HPLC, MS, thiab NMR

Zoo sib xws hauv cov qauv, solubility, thiab bioactivity

Siab batch stability

2. Qib tshawb fawb

Purity kwv yees li 95% -98%, siv rau kev sim tshuaj biochemical, cell, los yog tsiaj txhu.

Nta:

Tus nqi qis dua

Ua tau raws li qhov xav tau ntawm kev tshawb fawb feem ntau

Zoo structural controllability

3. Muaj thiab Formulation Development Qib

Purity kwv yees li 90%–95%, feem ntau yog siv rau thaum ntxov- kev tshawb fawb txog kev tsim qauv, kev sim ua ntej, thiab pov thawj- ntawm - theem tswv yim.

Nta:

Tus nqi qis tshaj

Haum rau cov kev sim yooj yim uas purity tsis tseem ceeb

3. Physicochemical Properties

Raws li tetrapeptide, Epitalon muaj cov yam ntxwv ruaj khov ntawm luv peptides. Nws cov khoom physicochemical yog raws li nram no:

1. Molecular Formula thiab Molecular Luj

Molecular Formula: C₁₄H₂₂N₄O₉ (raws li tetrapeptide qauv)

Qhov Nruab Nrab Molecular Luj: 390.35 g / mol Nws cov qauv peptide luv luv ua rau muaj qhov hnyav me me thiab muaj peev xwm nthuav dav.

2. Solubility

Yooj yim soluble hauv dej

Muaj zog soluble nyob rau hauv diluted saline thiab buffer daws

Soluble tsis zoo hauv ethanol, acetone, thiab ethers

Nws cov dej solubility ua rau nws haum rau feem ntau lub neej kev tshawb fawb kev sim tshuab.

3. Kev ruaj ntseg

Epitalon nthuav tawm kev ruaj ntseg zoo hauv nws cov hmoov lyophilized:

Kev ua tau zoo ntev - lub sijhawm khaws cia hauv qis - qhov kub thiab txias, ib puag ncig qhuav

Kev ua siab ntev ntawm luv luv - lub sij hawm raug rau chav tsev kub

Txawm li cas los xij, hauv kev daws teeb meem, nws raug rau lub teeb thiab hydrolysis, xav tau tub yees siv.

4. pH Compatibility

Kev ruaj ntseg siab nyob rau hauv pH ntau ntawm 4-8, muaj kev nyab xeeb rau cov kab mob biochemical.

4. Cov tsos thiab xim yam ntxwv

High - zoo Epitalon raw khoom hmoov muaj cov yam ntxwv hauv qab no:

1. Xim

Dawb rau tawm -dawb hmoov

Qhov ze ntawm cov xim yog dawb huv, qhov qis dua cov ntsiab lus impurity feem ntau yog.

Lub xub ntiag ntawm daj, greyish -dawb, los yog granular lumps feem ntau qhia tau hais tias tsis tsim nyog cia los yog muaj oxidized impurities.

2. Kev tshwm sim

Fine, uniform, thiab tsis muaj lumps

Qhuav rau qhov kov, nrog me ntsis kev xav (tus yam ntxwv ntawm peptide hmoov)

Feem ntau nyob rau hauv lyophilized hmoov daim ntawv kom muaj kev ua si thiab stability

5. Qhov zoo (los ntawm kev tshawb fawb thiab cov khoom siv raw)

1. Cov qauv luv luv, tsis tshua muaj kev sib xyaw ua ke nyuaj

Ua los ntawm plaub cov amino acids, cov saw peptide luv luv, ua rau cov khoom lag luam siab thiab muaj zog batch - rau -batch reproducibility, uas yog qhov zoo rau cov qauv tsim.

2. Zoo heev stability, yooj yim cia thiab thauj

Lyophilized hmoov tuaj yeem khaws cia rau lub sijhawm ntev hauv qhov chaw txias, qhuav thiab tsis yooj yim degraded, ib qho txiaj ntsig tseem ceeb hauv kev txhim kho biopharmaceutical.

3. Yooj yim soluble hauv dej, yooj yim kov

Nws cov dej solubility zoo ua rau nws tsim nyog siv rau hauv kev sim ntawm tes, protein-cov kev tshawb fawb txog kev sib tshuam ntawm cov protein, thiab qhia txog txoj kev tshawb nrhiav.

4. Kev tswj tau siab, zoo -txhais thaj tsam kev nyab xeeb

Cov qauv peptide synthesized tau zoo- txhais thiab tsis muaj tsiaj cov ntaub so ntswg impurities, uas yuav pab kom muaj kev ruaj ntseg ntawm kev sim hloov pauv.

6. Cov txheej txheem tsim khoom (Cov lus piav qhia luv luv)

Epitalon feem ntau yog npaj siv cov khoom siv -theem peptide synthesis (SPPS), nrog cov nram qab no ntws:

Siv cov resin ua cov khoom siv -phase carrier

Gly, Asp, Glu, thiab Ala tau txuas ntxiv (Fmoc tiv thaiv zoo)

Coupling cov neeg sawv cev raug ntxiv ntawm txhua kauj ruam los txhawb kev tsim peptide daim ntawv cog lus

Tom qab ua tiav, cov saw peptide yog cleaved

Cov pab pawg tiv thaiv raug tshem tawm

Purification (feem ntau yog siv RP-HPLC)

Lyophilization ua hmoov

Cov txheej txheem no yog paub tab thiab txhim khu kev qha, ua kom muaj kev lag luam tas mus li.

7. Zoo thiab kev kuaj

High-zoo Epitalon yuav tsum tau lees paub los ntawm cov kev sim hauv qab no:

HPLC: purity nrhiav pom

MS: Kev lees paub qhov hnyav Molecular

NMR: Kev txheeb xyuas qhov sib xws

Cov ntsiab lus noo noo (Karl Fischer txoj kev)

Residual solvent (GC nrhiav pom)

Cov khoom qib siab - feem ntau muab Daim Ntawv Pov Thawj Kev Ntsuam Xyuas (COA) thiab cov ntaub ntawv taug qab batch.

8. Kev cia, ruaj khov, thiab kev thauj mus los

Thaum Epitalon hmoov ruaj khov, yuav tsum tau khaws cia kom zoo:

1. Kev cia khoom

Ntev - lub sij hawm cia ntawm -20 degree

Nruab nrab- lub sij hawm cia ntawm 2–8 degree

Tiv thaiv los ntawm lub teeb, noo noo, thiab oxidation.

2. Lub Xeev daws teeb meem

Npaj rau kev siv tam sim ntawd xwb.

Yog tias tsim nyog, khaws cia ntawm -20℃thiab tsis txhob rov qab khov-thaw mus.

rhiab heev nyob rau hauv kev daws teeb meem; yuav tsum tau siv rau lub sijhawm luv luv.

3. Kev thauj mus los

Lyophilized hmoov yog tsim rau kev xa khoom thoob ntiaj teb; nws tuaj yeem tiv taus luv luv - lub sij hawm xa tuaj ntawm qhov kub thiab txias.

9. Daim Ntawv Qhia Cov Lus Qhia (Kev Tshawb Fawb Txog Kev Tshawb Fawb)

Epitalon feem ntau siv hauv cov chaw tshawb fawb hauv qab no:

Kev sim biochemical ntawm cov protein-protein kev sib cuam tshuam

Cov qauv muaj feem xyuam rau cov txheej txheem kev laus ntawm tes

Kev tshawb fawb txog cov qauv -kev ua haujlwm sib raug zoo ntawm peptides

Kev tshuaj xyuas ntawm pineal caj pas-txog txoj kev taw qhia

Kev tshawb nrhiav thiab thev naus laus zis validation ntawm cov qauv tshiab

Nws cov qauv yooj yim thiab cov khoom ruaj khov ua rau nws ua qauv classic rau kev tshawb fawb peptide luv.

10. Cov lus xaus

Epitalon, luv - saw, siab- zoo peptide tsim los ntawm tetrapeptides, tuav tus nqi tseem ceeb hauv kev tshawb fawb thiab biochemistry. Nws ruaj khov physicochemical zog, zoo solubility, thiab yooj yim txheej txheem ntau lawm txheej txheem ua rau nws ib tug heev nrhiav-tom qab cov cuab yeej kuaj sim.

Los ntawm cov tsos, xim, thiab impurity tswj mus rau synthetic txoj kev, cia txoj kev, thiab kev siv, Epitalon tau tsim ib tug paub tab kev lag luam saw.